Clinical possibilities of flow cytometry in hodgkin’s lymphoma

Hodgkin’s lymphoma (HL) is a unique of b-cell origin, the tumor cells which have lost expression main antigens. The standard immunophenotype substrate, according to immunohistochemical studies, characterized by such markers as CD15, CD30 and PAX-5, while negative CD3, CD19 in most cases also CD20 CD45 (or express this antigen rather weakly). knowledge gained recent years has increased effectiveness diagnosis, prognosis treatment LH. Flow cytometry, method immunophenotyping classical LH, was practically not used due difficulty distinguishing single reed–sternberg–berezovsky (RSB) microenvironment (reactive background), very rich cellular elements represented T cells, B eosinophils, histiocytes plasma cells. However, past, several studies successfully attempted identify rsb using multiparameter flow cytometry during aspiration with thin needle or biopsy lymph node tissue confirm supplement staining primary diagnosis. Taking into account characteristic RSB may become an additional diagnostic LH future. In review, we summarize data on possibility clinical option diagnosis